Abstract: In this paper, three kinds of snake venoms and four kinds of enzymes (phospholipase A₂, fibrinolytic enzyme, arginine esterase and L-amino acid oxidase) isolated from the snake venom were analyzed. As the snake venom was different, the MALDI/TOF/MSshowed difference. The MALDI/TOF/MSdetermination results could be affected by the concentrations of snake venom enzymes. And the mechanisms of desorption and ionization was also given in this study. By using MALDI/TOF/MSwe obtained the accurate molecular weights and homogeneities of the enzymes. The apparent characteristics of the positive MALDI/TOF/MSof enzymes composed by two subunits were also given out. The results showed that MALDI/TOF/MSis an effective analytic method for discovering new components from snake venom complexes. And it is reliable to use this method to determine the molecular weights and purifies of protein molecules.

Key words: Snake venoms, Enzymes, MALDI/TOF/MS