Affinity Chromatography Based on the Oligohistines as the Ligands

Abstract

Abstract: Aseries of affinity media were synthesized by using the oligohistines, His-Gly, (His)₃-Gly and (His)₅-Gly as the ligands and the home made monosized, non-porous copolymer(CL-PGMA) beads containing active epoxide groups as the support. The affinity chromatographic behavior between IgGand the oligohistines was investigated at pH3.0, 4.5, 7.0 and 10.0. It showed the specific interaction at pH4.5. The dissociation constants of IgGand oligohistine media(1H, 3H, 5H) were 3.037×10^-6 , 2.459×10^-6 and 1.905×10^-6 L/mol, respectively. The dissociation constants decreased with increasing the number of histine-residue in oligopeptide chains.

Key words: IgG, Oligohistine, HPAC, Dissociation constant, Synthesis of peptide in solid phase

CLC Number:

O657.7

TrendMD:

YU Xiao, ZHAO Rui, FANG Mei, XIONG Shao-Xiang, SU Tian-Sheng, LIU Guo-Quan . Affinity Chromatography Based on the Oligohistines as the Ligands[J]. Chem. J. Chinese Universities, 2000, 21(4): 532.

[1]	CHU Yan-Qiu* , DAI Zhao-Yun, JIANG Gong-Yu, ZHENG Song-Bai, CHEN Xi....... Studies on Non-covalent Complexes of Glutathione with L-Aromatic Amino Acids by Electrospray Ionization Mass Spectrometry [J]. Chem. J. Chinese Universities, 2009, 30(5): 869.
[2]	MO Zhi-Hong*, HUANG Hong-Ji, QIAN Jun-Zhen, ZHU Li-Hua. Piezoelectric Immunosensor Detection of IgG Based on Nanoprobe Immunoagglutination [J]. Chem. J. Chinese Universities, 2007, 28(4): 649.
[3]	WU Ya-Lan, LI Long-Di, LIU Jia-Ming, ZHU Guo-Hui . Solid Substrate Room Temperature Phosphorescence of Eosin-5-isothiocynate and Application in Immune Analysis Using CaCl₂ as an Enhancer [J]. Chem. J. Chinese Universities, 2005, 26(1): 49.
[4]	ZHU Hai-Lin, CHEN Heng-Wu, ZHOU Yong-Lie. Determination of IgG in Human Serum by Sequential Injection Renewable Surface Fluorescence Immunoassay System with a Chip-based Flow-through Cell [J]. Chem. J. Chinese Universities, 2004, 25(S1): 151.
[5]	WEI Jiao, FANG Hui, KONG De-Ling, YU Yao-Ting. Studies on Adsorption Mechanism of TNFα with IgG Ligand [J]. Chem. J. Chinese Universities, 2004, 25(7): 1277.
[6]	LIN Peng, ZHENG Hong, YANG Huang-Hao, YANG Wei, ZHANG Chang-Gong, XU Jin-Gou . Enzyme-linked Fluorescence Immunoassay for Human IgG by Using a pH-Sensitive Phase Separating Polymer as Carrier [J]. Chem. J. Chinese Universities, 2003, 24(7): 1198.
[7]	DUAN Jing-Hua, WANG Ke-Min, TAN Wei-Hong, HE Xiao-Xiao, HE Chun-Mei, LIU Bin, LI Du, HUANG Shan-Sheng, YANG Xiao-Hai, MO Yuan-Yao. A Study of a Novel Organic Fluorescent Core-shell Nanoparticle [J]. Chem. J. Chinese Universities, 2003, 24(2): 255.
[8]	FANG Can-Liang, ZHAO Rui, LIU Yang, YU Xiao, XIONG Shao-Xiang, LIU Guo-Quan . Screening of Affinity Peptide Ligands from Combinatorial Peptide Libraries [J]. Chem. J. Chinese Universities, 2003, 24(1): 52.
[9]	LIU Jia-Ming, FU Yan, YU Bing-Bin, LI Long-Di . Determination of IgG in Human Serum by Solid Substrate Room Temperature Phosphorescence-Immunoassay [J]. Chem. J. Chinese Universities, 2001, 22(10): 1645.
[10]	YANG Guang-Fu, LIU Hua-Yin, YANG Hua-Zheng . Acidity Constants of Herbicidal Sulfonylureas and Sulfonamides from Potentiometric Determinations in Aqueous Dimethyl Sulfoxide [J]. Chem. J. Chinese Universities, 1999, 20(12): 1883.
[11]	WANG Qiong-E, ZHUANG Hui-Sheng, ZHANG Fan. Studies on the Label of Antibody by a New Reagent N-(β-Carboxypropionyl)isoluminol [J]. Chem. J. Chinese Universities, 1996, 17(7): 1035.
[12]	Su Zhong-min, Huang Zong-hao, He Yue-hua, Gao Feng. A Quantum Chemistry Study on the Influence of Hydrogen Bond on the Acidity of Salicylic Acid [J]. Chem. J. Chinese Universities, 1991, 12(9): 1259.
[13]	Jiang Xiang-shun, Liang Chun-yu, Qin Mei-jie. Studies on Standard pH Reference Values in Mixed Solvents--The System of KHPh(0. 05 mol/kg)+KCl+Isopropanol+Water from 278. 15 to318. 15 K [J]. Chem. J. Chinese Universities, 1991, 12(9): 1234.
[14]	Wang Chang-shou, Tao Zu-yi . Intrinsic Dissociation Constant of Weakly Basic Anion Exchange Resin [J]. Chem. J. Chinese Universities, 1991, 12(5): 664.
[15]	Yang Pin, Ma Gui-bin, Zhao Hong-wei . The Coordination Interaction of a-Globulin with Rare Earth Metal Ion Gadolinium (Ⅲ) [J]. Chem. J. Chinese Universities, 1991, 12(5): 577.

Affinity Chromatography Based on the Oligohistines as the Ligands

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments