Abstract: The construction of glucose biosensor by immobilization of glucose oxidase(GOD) on the surface of hydrophilic Au and hydrophobic SiO₂ nanoparticles is reported, and polyvinyl butyral(PVB) is used as auxiliary immobilization enzyme matrix. The data of the current response of enzyme electrodes containing hydrophilic Au and hydrophobic SiO₂ nanoparticles to glucose are obtained. The experiments show that the nanoparticle would have a very strong enhancement effect on the GOD electrode response sensitivity. A mechanism for the behavior of the different kind of nanoparticles is proposed. The experiment and theory proved the function of nanoparticles in immobilization enzyme. This report provides experimental and theoretical basis for preparing the useful glucose biosensor.

Key words: Immobilization enzyme, GOD, Hydrophilic Au particle, Hydrophobic SiO₂ particle