Chem. J. Chinese Universities ›› 2000, Vol. 21 ›› Issue (1): 62.

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Immobilization of STI on Polymerized Liposome by Inter Arm and Its Affinity Adsorption for Trypsin

ZHANG Wen-Tao1, YU Kai2, SUN Yan2   

  1. 1. Department of Chemistry, Neimenggu Nationality Teachers College, Tongliao 028043, China;
    2. Department of Chemical Engineering, Tianjin University, Tianjin 300072, China
  • Received:1998-12-31 Online:2000-01-24 Published:2000-01-24

Abstract: Soybean trypsin inhibitor(STI) was immobilized on polymerized liposome of pentacose-10,12-diene-1-ol-phospho ethanolamine by diglycolic anydride, glutaraldehyde and 1,4-butanediol diglycidyl ether. In the course of precipitation, 20% to 60% trypsin does cleavage from PL-STI and shows PL-STI can be used as the affinity precipitation adsorbent of enzyme.

Key words: Polymerized liposome, Trypsin, Affinity precipitation

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