Chem. J. Chinese Universities ›› 1999, Vol. 20 ›› Issue (S1): 514.

• Biological Analysis • Previous Articles     Next Articles

Comparison of Antigen/Antibody Immobilization Methods in Immunoassay

LIANG Ji-Xnan1, LI Qing-Ge2, WEI Lin2   

  1. 1. Cancer Research Center, Xiamen Universily, Xiamen, 361005, P. R. China;
    2. The State Laboratory for Tumor Cell Engineering, Xiamen Universily, Xiamen, 361005, P. R. China
  • Online:1999-12-31 Published:1999-12-31
  • Supported by:

    Xiamen Talent Biotech, Co. Ltd is greatly acknowledged for kindly providing antigens and antibodies.

Abstract:

Immunoreagents, ether antibodies or antigens, can be immobilized onto solid phase in one of three different ways:adsorption to predominantly hydrophobic surfaces, covalent attachment to activated surface groups, and specific binding between a molecule immobilized by either of the above two methods, which in turn is coupled to a ligand.

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