Abstract:

The dye-binding assay has become an important method for protein analysis,primarily due to its convenience and high sensitivity. Only one simple reagent such as triphenylmethan-dye is required, and color development is generally rapid. The aim of this work is to develop a superior visual method with a high sensitivity for proteins in an aqueous solution. Here we show a novel protein assay based on the membrane filtration of the ternary associates and subsequent color change of the chromophore.