Abstract:

It has been recognized for half a century that stagnant mobile phase mass transfer is a dominant limitation in liquid chromatography. The resolution is seriously affected at high mobile phase velocity. One approach to solve this problem is to eliminate the pores. Through the use of 1~2 μm non-porous particles it has been possible to carry out protein separations an order of magnitude faster,albeit at the expense of diminished loading capacity and high operating pressure. A second alternative is to cause liquid to flow through the particles. Because convective transports is much more rapid than that achieved by diffusion, these materials can also be used at an order of magnitude higher velocity for mobile phase in protein separations.