CJCU

Chem. J. Chinese Universities ›› 1999, Vol. 20 ›› Issue (7): 1055.

• Articles • Previous Articles     Next Articles

A Rapid Method for the Determination of Molar Ratio of Fluorochrome to Protein Techniques by Fluorescence Anisotropy Detection

LI Dong-Hui1, YE Dong1, ZHU Qing-Zhi1, FANG Ying2, XU Jin-Gou1   

  1. 1. Department of Chemistry and the Key Laboratory of Analytical Science of MOE, Xiamen University, Xiamen, 361005;
    2. Anticancer Research Center, Xiamen University, Xiamen, 361005
  • Received:1998-07-18 Online:1999-07-24 Published:1999-07-24

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Abstract: The Determination of molar ratio of fluorochrome to protein is an important part in fluorescent antibody techniques. The conventional method is time consuming and with troublesome manipulations. Arapid homogeneous method based on the anisotropy change of the fluorochrome after reacting with protein was presented here. In our experiments, fluorescein isothiocyanate(FITC) and bovine serum albumin(BSA) were chosen to form the FITC/BSA conjugate. Aseries of solutions containing the two components were prepared and allow to react according to the standard procedure. analysis. The results were in good agreement.

Key words: Fluorescent antibody technique, Fluorescence polarization, Fluorescence anisotropy, Homogeneous phase

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