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Chem. J. Chinese Universities ›› 1999, Vol. 20 ›› Issue (3): 369.

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Studies on the Determination of Superoxide Anion Radical(O2-·) and Superoxide Dismudase(SOD) Activity by Flowinjection Spectrophotometry

DU Ming1, LIANG Fang-Zhen1, TANG Bo1, SHEN Han-Xi2   

  1. 1. Department of Chemistry, Shandong Normal University, Jinan, 250014;
    2. Department of Chemistry, Nankai University, Tianjin, 300071
  • Received:1998-05-11 Online:1999-03-24 Published:1999-03-24

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Abstract: Four thiocarbohydrazones of 1,5-bis(salicylidene)thiocarbohydrazone(BSTZ), 1,5-bis(phydroxybenzaldene)thiocarbohydrazone (BHBTZ), 1,5-bis(mmethoxyphydroxybenzaldene)thiocarbohydrazone(BMHBTZ) and 1,5-bis(mmethoxysalicylidene)thiocarbohydrazone(BMSTZ) were synthesized. BHBTZ and BMHBTZ can be oxidated by superoxide anion radical(O2-·) and H2O2.Based on the reaction, the determination of O2-·by flow injection spectrophotometry was developed. The linear range of O2-·concentration is from 4.5×10-7mol/Lto 1.5×10-6mol/L. The detection limit is 1.5×10-7mol/L. The relative standard deviation is 5.23%. Coexist substances in many biological samples have no interference. The method can also be used to determine the activity of superoxide dismudase(SOD).

Key words: Thiocarbohydrazone, Superoxide anion radical, Superoxide dismudase activity, Flow injection spectrophotometry

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