Studies on the Solution Conformation of Angiotensin Ⅱ

Chem. J. Chinese Universities ›› 1998, Vol. 19 ›› Issue (11): 1758.

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Studies on the Solution Conformation of Angiotensin Ⅱ

WANG Rui¹, HU Xiao-Yu¹, MA Ya-Ping¹, Li Du²

1. Biochemistry Laboratory of Biology Department, State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, 730000;
2. Department of Chemistry, Brigham Young University, Provo, Utah, United States

Received:1997-11-12 Online:1998-11-24 Published:1998-11-24

Abstract

Abstract: Human angiotensin (AngⅡ) was obtained by solid phase method. Its solution conformations in D₂Oand DMSOd₆ were examined by 2D NMRtechnique. The dihedral angle constraint, distance constraint, hydrogen bond constraint were made based on coupling constants, ROE effect, temperature coefficient of chemical shifts. It is concluded that Ang Ⅱ exists in D₂Oand DMSOd₆ as closed similar conformations that is random coil with negligible content of α helix, 3₁₀ helix, β turns, γ turns, parallel and antiparallel β sheet. The conformation in D₂Owas not sensitive to temperature.

Key words: AngⅡ, Solid phase peptide synthesis, 2D NMR, Solution conformation of peptide

CLC Number:

Q5.71

TrendMD:

WANG Rui, HU Xiao-Yu, MA Ya-Ping, Li Du. Studies on the Solution Conformation of Angiotensin Ⅱ[J]. Chem. J. Chinese Universities, 1998, 19(11): 1758.

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Studies on the Solution Conformation of Angiotensin Ⅱ

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