Abstract: The binding reaction and resonant luminescence spectra of ethyl violet with ctDNAhave been studied. In the pHrange of 5.0—10.0 ethyl violet exhibited a maximum of absorption at 595 nm. Anew absorption peak at 507 nm appeared when ctDNAwas added. It showed that a binding product of ethyl violet with DNAformed. The product emitted a sharp resonant luminescence peak at 510 nm. The intensity of resonant luminescence was found to be dependent on the concentration of DNA. The linear range of the calibration graph was 0—500 ng/mL. The limit of detection(3 σ) was 1.54 ng/mL. Anew method of resonant luminescence for the determination of trace DNAwas established with high sensitivity and selectivity.

Key words: Ethyl violet, Deoxyribo nucleic acid, Resonant luminescence spectrometry