Immobilization of α-Chymotrypsin and its Application to Resolution of DL-Phenylalanine

Chem. J. Chinese Universities ›› 1997, Vol. 18 ›› Issue (8): 1388.

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Immobilization of α-Chymotrypsin and its Application to Resolution of DL-Phenylalanine

LIU Li-Jian, YANG Ping, ZHUO Ren-Xi

Department of Chemistry, Wuhan University, Wuhan 430072

Received:1996-06-14 Online:1997-08-24 Published:1997-08-24

Abstract

Abstract: α-Chymotrypsin has been immobolized on porous silica beads. The activity,opti-mum temperature and pHof the immobilized chymotrypsin as well as its Micllaelis constant have also been determined. its stabilities to heat, acid, base, methanol and urea are in-creased remarkably. It has been applied to resolving DL-phenylalanine continuously more than two months with good performance. L-Phenylalanine is obtained in a yield of 90%, and its optical purity reaches as high as 96%.

Key words: α-Chymotrypsin, Immobolization, L-Phenylalanine, Optical resolution

CLC Number:

O631.9

TrendMD:

LIU Li-Jian, YANG Ping, ZHUO Ren-Xi . Immobilization of α-Chymotrypsin and its Application to Resolution of DL-Phenylalanine[J]. Chem. J. Chinese Universities, 1997, 18(8): 1388.

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Immobilization of α-Chymotrypsin and its Application to Resolution of DL-Phenylalanine

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