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Chem. J. Chinese Universities ›› 1997, Vol. 18 ›› Issue (7): 1041.

• Articles • Previous Articles     Next Articles

A Fiber-optic Biosensor Based on Visible Absorption for the Dtermination of Lactate Dehydrogenase Activity

WANG Ke-Min1, LIN Zhao-Hui1, CAI Wei1, Chan W. H.2, Lee Albert W. M.2   

  1. 1. College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082;
    2. Department of Chemistry, Hong Kong Baptist University, Hong Kong
  • Received:1997-04-04 Online:1997-07-24 Published:1997-07-24

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Abstract: Lactate dehydrogenase activities can be determined by using a fiber-optic biosen-sor based on visible absorption.In this method two dehydrogenate procedures were catalyzedby lactate dehydrogenase and diaphorase respectively, and coenzyme I (NAD+/NADH) wasintroduced into the two enzyme reactions as an electron transfer.The first dehydrogenateprocedure is for the recognition of lactate dehydrogense and the second one will show achange of absorbance- The sensing element of the biosonsor consists of draphorase covalencebound on the tip of the fiber bundle.Alinear response of the biosensor was obtained in therange of 0-400 U/Lof lactate dehydrogenase with a detection limit of 48 U/L- This biosen-sor has been used to determine lactate dehydrogenase activities in human serum.

Key words: Fiber-optic biosensor, Dehydrogenase activity, Lactate dehydrogenase, Di-aphorase, Coenzyme I

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