Abstract: In this paper, a novel fluorescence method for the determination of vitamin B2 and vitamin B6 has been established using three-dimensional fluorescence spectrometry by detect-ing the total fluorescence intensity. The sensitivity of the method, the ratio of signal to blank noise, is nearly 130 and 310 times better than that of the conventional peak intensity method for the determination of vitamin B2 and vitamin B6, respectively. This method has been successfully used to determine vitamin B2 and vitamin B6 simultaneously.

Key words: Three-dimensional fluorescence spectrometry, Vitamin B2, Vitamin B6