Studies on the Reaction Between Laccase and 3,4,5-Trihydroxybenzoic Acid by Microcalorimetry

Abstract

Abstract: The reactions between laccase and 3,4,5-trihydroxybenzoic acid have been studied by LKB-2107 batch microcalorimetry system at different temperatures and pH = 7.4.The rnolar reaction enthalpy (Δ_rH_m), the Michaelis constant (K_m), the rate constant (k₂), the laccase activity (E_A), the binding energy (ΔG₀), the activation Gibbs free energy (ΔG_≠^T),the actlvation energy(E_a) and the activation entropy (ΔS_≠^T) have been determined.The results have been discussed from changes in free energy by using the transition state theory.The activation entropy (ΔS_≠^T ＜ 0) indicated that enzyme-substrate/transition structure is　bound more orderly than enzyme-substrate complex.

Key words: Laccase, Microcalorimetry, Transition state,3,4,5-Trihydroxybenzoic acid

TrendMD:

WANG Tian-Zhi, WU Ding-Quan, WAN Hong-Went, QU Song-Sheng, DU Yu-Min. Studies on the Reaction Between Laccase and 3,4,5-Trihydroxybenzoic Acid by Microcalorimetry[J]. Chem. J. Chinese Universities, 1997, 18(5): 786.

References

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