Abstract: Anovel method "pre-polymerization" for production of cross-linked polyacrylamide gel filled-capillaries was studied in detail.Many factors of this method which contribute to the ratio of success for producing the gel-filled capillaries were discussed.i.e.,characters and pureness of Acr and Bis, use level of TeMEDand AP, effect of pHvalue and other characters of buffer, time of polymerization, diameter and length of capillaries, and conditions of preservation and so on.This method was demonstrated to be applicable to the production of gel-filled capillaries with wide varieties of gel composition, capillary diameters and capillary lengh.Over 9O% ratio of success for producing the gel-filled capillaries was obtained with this method.Retroreflective laser beams interference phase-shift detection was used for on-column detection of proteins in cross-linked polyacrylamide gel capillary electrophoresis (CPAGCE) firstly.For ovalbumin, 4.2 ×10^-7 mol/L(S/N=2) of detection limit was measured and 1.66 ×10₅ theoreticaｌplates was observed on the home-made CEsystem.It should prove attractive for a number of applications in separation of biological macromolecule samples with the gel-filled capillaries produced by the method of pre-polymerizatlon.

Key words: Cross-linked polyacrylamide, CGE, Prepolymerization