Abstract: The preliminary result of the yeast-lipase purified and separated by the reversemicelles system of AOT/iso-octane is reported in the paper. The phase transfer mechanismof the static interaction in the process of the phase transfer of reverse micelles as well as thehydroplic interaction was studied based on the extractive efficiency of lipase and the recoveryefficiency of protein affected by pHvalue of water phase and salt concentration. Theinteraction between enzyme molecules and reverse micelles was reduced and the recovery efficiencyof protein and enzyme activity was increased significantly when deoxycholic acid was addedinto the reverse micelles of AOT/iso-octane as the biological surfactant. The relative activityof lipase recovered was increased by 4%～9% compared with origin enzyme aqueous in therange of pHinvestigated.

Key words: Reverse micelles, Phase transfer, Yeast-lipase, Deoxycholic acid