Abstract: Bioconcentration curves of 2, 4-DNT in carps (whole fish, liver, intestine) wereinvestigated using semistatic systems. For whole fish, its curve could be described as agentle peak which began with a rise in concentration to steady state, then declined and reachedlower level followed by another steady state. For liver and intestine, their curves bothcontained two successive peaks. Bioconcentration factors (BCFs) of 2, 4-DNTin whole fish atthe first and second steady state were 9. 15 and 4. 15 respectively. By logarithmic ploting,two straight lines with different slopes 3. 6 and 0.1 d^-1 were measured for elimination. Twometabolites were isolated by HPLCas 4-amino-2-nitrotoluene and 2,4-diaminotoluene, theirretention times were 23.1 and 8. 8 min respectively. Aone-compartment model was set up tosimulate the uptake, in which biotransformation adhered to delayed enzyme-catalyticlogarithmic kinetics. Modeling curve was in accord with measured values.

Key words: 2,4-Dinitrotoluene(2,4-DNT), Carps, Peaky bioconcentration curve