Chem. J. Chinese Universities ›› 1996, Vol. 17 ›› Issue (7): 1035.

• Articles • Previous Articles     Next Articles

Studies on the Label of Antibody by a New Reagent N-(β-Carboxypropionyl)isoluminol

WANG Qiong-E, ZHUANG Hui-Sheng, ZHANG Fan   

  1. Depatment of ChemiStry, Fuzhou University, Fuzhou 350002
  • Received:1995-06-21 Online:1996-07-24 Published:1996-07-24

Abstract: Anew reagent N-(β-carboxypropionyl)isoluminol (CPIL)is used to label sheep anti-human IgG(SaHIgG).The labeled antibody was stable and could be detected at least down to 10-13mol/L,The molar incorporation ratio is estimated to be 0.28 mol of CPILper mol of SaHIgG.There are no apparent changes in the immunoreactivity of the labeled SaHIgGand in the quantum efficiency of the CPILattached to the protein.

Key words: Chemiluminescence immunoassay, N-(β-carboxypropionyl)isoluminol, Sheep anti-humun IgG

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