Chem. J. Chinese Universities ›› 1996, Vol. 17 ›› Issue (7): 1035.
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WANG Qiong-E, ZHUANG Hui-Sheng, ZHANG Fan
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Abstract: Anew reagent N-(β-carboxypropionyl)isoluminol (CPIL)is used to label sheep anti-human IgG(SaHIgG).The labeled antibody was stable and could be detected at least down to 10-13mol/L,The molar incorporation ratio is estimated to be 0.28 mol of CPILper mol of SaHIgG.There are no apparent changes in the immunoreactivity of the labeled SaHIgGand in the quantum efficiency of the CPILattached to the protein.
Key words: Chemiluminescence immunoassay, N-(β-carboxypropionyl)isoluminol, Sheep anti-humun IgG
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WANG Qiong-E, ZHUANG Hui-Sheng, ZHANG Fan. Studies on the Label of Antibody by a New Reagent N-(β-Carboxypropionyl)isoluminol[J]. Chem. J. Chinese Universities, 1996, 17(7): 1035.
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http://www.cjcu.jlu.edu.cn/EN/Y1996/V17/I7/1035