Solid Phase Peptide Synthesis of Designed Yeast Transcriptional Activator Ac [Q236,Q242]GCN4(226─252)─GGC─NH2 and Its Dimerization

Chem. J. Chinese Universities ›› 1996, Vol. 17 ›› Issue (5): 747.

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Solid Phase Peptide Synthesis of Designed Yeast Transcriptional Activator Ac [Q²³⁶,Q²⁴²]GCN4(226─252)─GGC─NH₂ and Its Dimerization

ZHANG Ruo-Heng, WANG He, XU Xiao-Jie, TANG You-Qi

Institute of Physical Chemistry, Peking University, Beijing 100871

Received:1995-03-01 Online:1996-05-24 Published:1996-05-24

Abstract

Abstract: Yeast transcriptional activator GCN4(226-252)-GGC-NH₂ and the designed Ac-[Q²³⁶,Q²⁴²)]GCN₄(226-252)-GGC-NH₂ aiming at altering GCN4 DNA-binding specificity was prepared by stepwise solid phase peptide synthesis.It was found that the yield of the peptide would be greatly improved when treated with dithiothreitol(DTT).The dimer was obtained by air oxidation, which would be used in the sequence-specific DNAbinding activity experiments.

Key words: Yeast transcriptional activator, Solid phase peptide synthesis, Air oxidation

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ZHANG Ruo-Heng, WANG He, XU Xiao-Jie, TANG You-Qi. Solid Phase Peptide Synthesis of Designed Yeast Transcriptional Activator Ac [Q²³⁶,Q²⁴²]GCN4(226─252)─GGC─NH₂ and Its Dimerization[J]. Chem. J. Chinese Universities, 1996, 17(5): 747.

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Solid Phase Peptide Synthesis of Designed Yeast Transcriptional Activator Ac [Q²³⁶,Q²⁴²]GCN4(226─252)─GGC─NH₂ and Its Dimerization

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