Abstract: The kinetic constants of different aromatic amines substrates catalyzed by HRPin the organic solvent and aqueous micelle system were compared by the method of fluorescence,It was found that the catalytic reactions by HRPin the aqueous micelle system took place on a strict hydrophilic/hydrophobic interface;Meanwhile,the enzymatic properties on the interface were preliminarily studied and the effect of the substrates with different extending solvent sites in the micelle on the reaction kinetic constants was discussed.

Key words: Horseradish perosidase, Aqueous micelle, Reaction microenvironment