Studies on Screening Immune Drugs by Linear Sweep Polarography

Chem. J. Chinese Universities ›› 1996, Vol. 17 ›› Issue (11): 1702.

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Studies on Screening Immune Drugs by Linear Sweep Polarography

KANG Xiao-Feng¹, SONG Jun-Feng², HU Yin-Hua²

1. Department of Chemistry and Chemical Engineering, Baoji College of Arts and Science, Baoji, 721007;
2. Institute of Electroanalytical Chemistry, Northwest University, Xian, 710069

Received:1995-12-26 Online:1996-11-24 Published:1996-11-24

Abstract

Abstract: The hemolytic activity of hemolysin was investigated by linear sweep polarography in this paper.Asensitive polarographic reduction wave of hemolysin was observed in 1/15 mol/L Na₂HPO₄～1/15 mol/L KH₂PO₄ buffer solution(pH=7.4).The peak potential was -1.82 V(vs.SCE).The peak height was linearly proportional to its biological activity in the ranges of 6.0×10^-6～5.3×10^-5 unit/mL and 5.3×10^-5～2.0×10^-3 unit/mL.According to the various effects of drugs on the formation of hemolysin antibody,a new method for screening immune drugs was established.This method is simple,rapid,and the assay of hemolysin activity is no longer dependent upon immune hemolysis process.The results are accurate and reliable.

Key words: Hemolysin, Polarography, Immune drug

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KANG Xiao-Feng, SONG Jun-Feng, HU Yin-Hua. Studies on Screening Immune Drugs by Linear Sweep Polarography[J]. Chem. J. Chinese Universities, 1996, 17(11): 1702.

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Studies on Screening Immune Drugs by Linear Sweep Polarography

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