Abstract: We have prepared a dodecapeptide, which was first extracted from Chinese rape pollen, by using solid-phase synthesis method.In order to study its structure function relation, seven peptide segments and five analogues were designed and synthesized. We have initially discussed their structures in solid state and different solvents using FTIRand CDspectra. In addition, their immunopromotive activies have been observed by E-rosette test, tumor necrosis factor(TNF) test and T-lymphocyte transformation test in vitro. The observed results show the dodecapeptide and some segments can cause the maturation of pig thymocyte in vitro and enhance the activity of TNF on murine tumor cells.

Key words: Rape pollen peptide, Solid-phase synthesis, Immune activity