CJCU

Chem. J. Chinese Universities ›› 1995, Vol. 16 ›› Issue (2): 184.

• Articles • Previous Articles     Next Articles

The Application of DTPA-Eu3+Complex to DNA Hybridization Assay

CI Yun-Xiang, CHEN Yong, LI Yuan-Zong, CNANG Wen-Bao   

  1. Deparment of Chemistry, Peking University, Beijing, 100871
  • Received:1994-04-07 Revised:1994-09-10 Online:1995-02-24 Published:1995-02-24

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Abstract: Here we reports a new detection method for λDNA through the system of target DNA/Bio-DNA probe/Avidin/ Bio-BSA(TG)-DTPA-Eu3+.Avidin was used as a bridge to link hybrids and Bio-BSA-DTPA-Eu3+or Bio-TG-DTPA-Eu3+.The carrier proteins were labeled with both biotin and DTPA-Eu3+.The analytical conditions were optimized.The influences of transamination of DNA probe by ethylene diamine and 1,6-hexane diamine as well as carrier proteins on hybridization assay were compared.The method has a high sensitivity(1pg/well) with good reproducibility(RSD=5.7%,n=6).

Key words: Hybridization assay, DTPA-Eu3+, Time resolved fluorometry

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