Effect of Trichloroacetic Acid on the Electronic Spectra of Bacteriorhodopsin

Chem. J. Chinese Universities ›› 1994, Vol. 15 ›› Issue (8): 1217.

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Effect of Trichloroacetic Acid on the Electronic Spectra of Bacteriorhodopsin

Fu Xue-Qi¹, WANG Jin-Dui², ZHU Jun², WANH De-Jun³, LI Tie-Jin³, CAO Jun-Wei⁴

1. State Key Laboratory of Enzyme Engineering, Jilin University, Changchun, 130023;
2. Department of Molecular Biology, Jilin University, Changchun, 130023;
3. Department of Chemistry, Jilin University, Changchun, 130023;
4. Department of Biology, Wuhan University, Wuhan

Received:1993-10-18 Revised:1994-03-02 Online:1994-08-24 Published:1994-08-24

Abstract

Abstract: Akind of protein denaturant─CCl₃COOH was used to change the microenvironment of bacteriorhodopsin gradually and resulted in bacteriorhodopsin denaturation finally.The surface photovoltage spectrum, adsorbance spectrum, circular dichroism spectrum and fluorescence spectrum were determined during the processes of changing the microenvironment of bacterorhodopsin, and the effect of trichloroacetic acid on the electronic spectra of bacteriorhodopsin was discussed.

Key words: Bacteriorhodopsin, Trichloroacetic acid, Electronic spectra, Surface photovoltage spectra

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Fu Xue-Qi, WANG Jin-Dui, ZHU Jun, WANH De-Jun, LI Tie-Jin, CAO Jun-Wei, WNAG Yu-Lin, CHEN Shu-Yun. Effect of Trichloroacetic Acid on the Electronic Spectra of Bacteriorhodopsin[J]. Chem. J. Chinese Universities, 1994, 15(8): 1217.

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Effect of Trichloroacetic Acid on the Electronic Spectra of Bacteriorhodopsin

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