CJCU

Chem. J. Chinese Universities ›› 1994, Vol. 15 ›› Issue (5): 672.

• Articles • Previous Articles     Next Articles

A Study of Nafion-Mercury Film Chemically Modified Electrode for the Determination of GPT

JIN Li-Tong, YE Jian-Shan, SHI Zhan-Ling, FANG Yu-Zhi   

  1. Department of Chemistry, East China Normal University, Shanghai, 200062
  • Received:1993-05-18 Revised:1993-10-04 Online:1994-05-24 Published:1994-05-24

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Abstract: α-Ketoglutaric acid is determined by use of the Nafion-mercury film chemically modified electrode (Nafion-Hg film CME). The activity of GPTin human serum is determined indirectly. The linear range for the determination of α-ketoglutaric acid is 5. 0×10-3—1. 0×10-6 mol.L. The lowest detection is 5.0×10-7mol/L.The range for the determinaion of GPTis 2-200 U/L. This method has a high sensitivity and resists with disturbance.Good results were obtained for tests of human blood samples.

Key words: Nafion-mercury film chemically modified electrode, Glutamic pyruvic transaminase, α-Ketoglutaric acid

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