CJCU

Chem. J. Chinese Universities ›› 1993, Vol. 14 ›› Issue (12): 1661.

• Articles • Previous Articles     Next Articles

Rapid Separation of Disaccharides by Capillary Zone Electrophoresis with Interference Refractive Index Detection

REN Ji-Cun1, HE Jin-Lan2, DENG Yan-Zhuo2, CHENG Jie-Ke1   

  1. 1. Department of Chemistry, Wuhan University, Wuhan, 430072;
    2. Departemtn of Analysis and Testing, Wuhan University, Wuhan, 430072
  • Received:1992-12-31 Revised:1992-03-12 Online:1993-12-24 Published:1993-12-24

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Abstract: Rapid separation of disaccharides by capillary zone electrophoresis in uncoating fused-silica column is presented.Using a pH 9.7, 0.1 mol/Lsodium tetraborate as the runing solution, underivatized disaccharides are transformed into negatively charged borate complexes.Detection is performed with an on-column laser interference refractive index detector (made in home).Four disaccharides are separated by CZEwithin 10 min.The effects of pH, sodium tetr-borete concentration and organic additives on the separation of disaccharides are discussed.

Key words: CZE, Laser interference, Disaccharides

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