Proteinase Fluorogenic Substrates Containing 7-Amino-4- methylcoumarin

Chem. J. Chinese Universities ›› 1993, Vol. 14 ›› Issue (11): 1556.

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Proteinase Fluorogenic Substrates Containing 7-Amino-4- methylcoumarin

ZHANG Ruo-Heng¹, JIA Shao-Juan¹, XU Xiao-Jie¹, TANG You-Qi¹, YANG Guang-Wei², NI Yi-Sheng²

1. Department of Chemistry, Peking University, Beijing, 100871;
2. Department of Biology, Peking University, Beijing, 100871

Received:1993-02-19 Revised:1993-07-10 Online:1993-11-24 Published:1993-11-24

Abstract

Abstract: The fluorogenic substrates have been used for the highly sensitive determination of chymotrypsin.The sensitivity is due to the fact that the leaving group, some fluorogenic amine, is highly fluororesent.In the present communication, we wish to report the design, synthesis and kinetic study of a series peptide amide substrates containing 7-amino-4-methylcoumarin.By calculating the enzyme's k_cat and K_m values for the substrates, the influence of amino acid sequence on the specificity of substrates was studied.Therefore, the best fluorogenic substrates for chymotrypsin, Suc-Ala-Ala-Phe-MCand Sue-Ala-Pro-Phe-MC, were found.

Key words: Fluorogenic substrate, Coumarin, Chymotrypsin

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ZHANG Ruo-Heng, JIA Shao-Juan, XU Xiao-Jie, TANG You-Qi, YANG Guang-Wei, NI Yi-Sheng. Proteinase Fluorogenic Substrates Containing 7-Amino-4- methylcoumarin[J]. Chem. J. Chinese Universities, 1993, 14(11): 1556.

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Proteinase Fluorogenic Substrates Containing 7-Amino-4- methylcoumarin

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