Studies on Photochemical Fluorimetric Method(Ⅸ)——Determination of Menadione in Vitamin K3 Injection by in situ Photochemical Fluorimetry

Abstract

Abstract: A method for determining menadione in vitamin K₃ injection by in situ photochemical fluorimetry is reported in this paper.The effects of reaction medium,photon wavelength,oxygen,and irradiation time on the photoreduction reaction are also tested and discussed.Alinear curve is obtained over the rang of 0～9.60μg/mLmenadione (r=0.999) and the determination limit is 2.5 ng/mLfor menadione (w=11).The relative standard deviation is 2.7%.The a-mount of menadione in vitamin K₃ injection determined by standard curve method and standard addition method is 94%～100% of marked amount.The recovery in standard addition test is 100%～107%.

Key words: Photochemical reaction, Fluorimetry, Vitamin K₃

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GUO Xiang-Qun, XU Jin-Gou, ZHAO Yi-Bing, CHEN Guo-Zhen . Studies on Photochemical Fluorimetric Method(Ⅸ)——Determination of Menadione in Vitamin K₃ Injection by in situ Photochemical Fluorimetry[J]. Chem. J. Chinese Universities, 1992, 13(9): 1199.

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Studies on Photochemical Fluorimetric Method(Ⅸ)——Determination of Menadione in Vitamin K₃ Injection by in situ Photochemical Fluorimetry

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