Abstract: Rhus vernicifera laccase catalyzes the oxidation of 2, 6-dimethylphenol(DMP) to 3, 3', 5,5' -tetramethyl-4,4' -diphenoquinone (TMDQ) in 0.1 rnol/Lphosphate buffer solution.The enzymatic oxidation of DMPis much faster at pH 8.0 and temperature 40～50℃.The reaction is a first-order and the rate constant (kobs) is 3.57× 10^-5 s^-1 when DMP concentration is in the range of 1.66 × 10^-4～9.92 × 10^-4 mol/L.The coexistent ferrocene (Fc-H) can more greatly promote the enzymatic oxidation of DMP.The possible mechanism of laccase-catalyzed oxidation of DMPand coexistent Fc-H was inferred.

Key words: Rhus vernicifera laccase, 2,6-Dimethylphenol, Ferrocene, Enzymatic promation oxidation, 3,3'5,5'-Tetramethyl-4,4'-diphenoquinone