CJCU

Chem. J. Chinese Universities ›› 1991, Vol. 12 ›› Issue (4): 469.

• Articles • Previous Articles     Next Articles

The Determination of Luminescence-Labelled Agent ABEI by Electrogenerated Chemiluminescence

Yao Qing1, An Jing-ru2   

  1. 1. Teaching and Kesearch Group of Chemistry, Fujian Medical College, Fuzhou, 350004;
    2. Department of Chemistry, Fuzhou University
  • Received:1989-12-14 Online:1991-04-24 Published:1991-04-24

PDF (PC)

Abstract: The electrogenerated chemiluminescence of ABEI and ABEI-HCG in H3BO3-KCl-Na2CO3buffer solution at pH 11. 06 was studied. Co(Ⅱ)(200～600 μg/L) is capable of catalyzing the ABEIluminescence when a pulse potential is applied to the electrodes. Antibody of HCGis combined with ABEIto obtain ABEI-IgG. The detection limit of ABEIand ABEI-IgGare 2. 0×10-11mol/Land 2. 1×10-10mol/L, respectively. The concentration of ABEI, from 1.0×10-10to 5.0×10-7mol/ Lis in a linear relationship with the intensity of light.

Key words: Electrogenerated chemiluminescent immunoassay, Aminobutyl ethylisoluminol, HCG

TrendMD: