Abstract: Four furostanol glycosides were isolated from 70% EtOH extract of the roots of Smi-lax Nigrescent Wang et Tang. Their structures were established on the basis of spectral and chemical methods. One of the glycosides is a new oligofurostanoside 26-O-β-D-glucopyranosyl-(25R)-furost-5, 20 (22)-diene-3β, 26-diol-3-O-[α-L-rhamnopyranosyl (1-2)]-β-D-glucopyra-noside and the others are, 26-O-β-D-glucopyranosyl-22-methoxy-(25R)-furost-5-ene-3.26-diol-3-O-[α-L-rhamnopyranosyl (1-2)]-β-D-glucopyranoside, methyl protodioscin and pseudopro-todioscin.

Key words: Smilax Nigrescens, Liliaceae family, Furostanol glycosides