Abstract: By means of NMR method, the modes and effects of the interaction between cis-diaquodiammineplatinum(Ⅱ) (AAP) and α-dipalmitoylglycerylphosphatidylcholine (DPPC) were studied to clarify the contribution of phospholipid-platinum reaction to the cisplatin-cell interactions. ¹H and ¹³C spectra show that in CDCl₃, as the result of DPPC-AAP reaction, the platinum binds to the head group of DPPC and induced a gauche to trans transformation in the glycerol moiety. The ¹H T₁ values of -N(CH₃)₃⁺,-(CH₂)_n- and -CH₃of DPPC in liposome form were determined at 65℃ after the reaction with AAP in D₂O for different time. The results indicate that the conformational change of phospholipid molecules and the changes in the set-up of the membranic molecules induced by the platinum binding may contribute to the cisplatin-cell interaction and are relevant to the anticancer activity and toxicity of cisplatin.

Key words: cis-Diaquodiammineplatinum (Ⅱ) (AAP), α-Eipalmitoylglycerylphosphatidylcholine (DPPC), Phospholipid-platinum reaction, Cisplatin-cell interaction