Abstract: The spherical copolymer of ethylstyrene with divinylbenzene for using as the packing material for Reversed-phase-HPLC has been prepared by a novel polymerization process. The 95% particle sizes of the polymers are within 3-7 μm. Some column parameters such as the permeability, efficiency and peak asymmetry were calculated. The excellent characters of this packing material for the separation of mixtures of amines, phenols and aromatic hydrocarbons were well demonstrated through several examples.

Key words: Spherical copolymer, Reversed-phase-HPLC, Column packing material