Solid Phase Synthesis of the Human Trypsin Inhibitor

Chem. J. Chinese Universities ›› 1990, Vol. 11 ›› Issue (8): 837.

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Solid Phase Synthesis of the Human Trypsin Inhibitor

Wang Ensi¹, Xu Jianping¹, Wang Zongmu¹, Li Wei¹, S. Funakoshi², H. Yajima²

1. Department of Molecular Biology, Jilin University, Changchun;
2. Faculty of Phamaceutical Science, Kyoto University, Kyoto, Japan

Received:1989-06-01 Online:1990-08-24 Published:1990-08-24

Abstract

Abstract: This paper describes synthesis of the human trypsin inhibitor (HTI) by the solid phase method in which both stepwise and segment condensation are involved. TMSOT_f-thioanisol/TFA have been used as the agent which cleaves HTIfrom the resin and removes all of the side chain protecting groups simultaneously. Crude HTIwas purified by affinity chromatography on anhydro- trypsinr sepharose and reverse phase HPLC, the total yield is 6.3%. The resulting product shows powerful trypsin inhibitory activity that near 97% of the activity of natural HTI.

Key words: Human trypsin inhibitor, Solid phase peptide synthesis, Trimethylsilyl trifluoromethane-sulf onate (TMSOT_f)

TrendMD:

Wang Ensi, Xu Jianping, Wang Zongmu, Li Wei, S. Funakoshi, H. Yajima. Solid Phase Synthesis of the Human Trypsin Inhibitor[J]. Chem. J. Chinese Universities, 1990, 11(8): 837.

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Solid Phase Synthesis of the Human Trypsin Inhibitor

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