Abstract: Nine compounds were isolated from the plant of Centaurea repens. By means of IR, UV, NMR, MS analysis and chemical reactions, the structure of nine compounds were elucidated as I : n-Nonacosane; Ⅱ : 1-Docosanol; Ⅲ : 3-Stigmastanol; Ⅳ : β-Sitosterol; Ⅴ: Stigmast-7-en-3-ol; Ⅵ: Tetratriacontanoic acid ( β: 2, 4-bis(o-tolylazo)naphth-l-ol; Ⅶ: β-sitosterol-3-β-D-glucoside and Ⅷ: Apigenin-5-oβ-D-glucoside. Ⅶ was isolated for the first time from the natural product. Its spectral data were analyzed in detail. The structure ofⅦ was also proved by chemical synthesis.

Key words: Gentaurea repens, Compositea, 2,4-Bis(o-tolylazo)-naphth-l-ol