Abstract: In this paper, resolution of DL-phc,PL-Phser, DL-Tyr, DL-Trp, DL-His etc was studied by liquid chromatography using L-Pro grafted resins coordinated with copper(Ⅱ) as stationary phase. Except DL-His, another amino acids mentioned above could be completely resolved and Dforms were eluated in front of Lforms Through determination of IR spectra of L-Pro-PGMA-Cu (Ⅱ) resin, its possible coordinated model of structure of resin and mechanism of resolution were presented.