Abstract: In this study, the bcsi condition of immobilized glucose oxidase using different supports and different methods was studied, and compared. An improved method of glucose oxidase immobilization method using glutaric dialdehyde was studied.One end of the glutaric dialdehyde molecule was first protected by diethanol-amine and the other end was linked to the carrier. Then the protected end was hydrolysed and glucose oxidase was linked to it. Clucose oxidase prepared according to the above method had the highest activity. The enzyme activity was 10% more than the didzo-reaction method and twice nigher than the polyacrylamide entrapment method.