Abstract: Using pyridoxine-tetraphenylboratc or pyridoxine-dipicrylamine ion-pair cpmplexes as active materials, PVCmembrane pyridoxine ion selective electrodes were prepared. The effects of various factors on the performances of electrodes were studied and the optimum condition of membrane preparation was found. The use of p-yridoine-dipicrylamine as active material and dibutylphthalate as solvent medium gives best electrode characteristics. This electrode shows Nernstian range from 1.0×10^-1Mto 1.0×10^-5M, with a slope of 61 mV/decade. The stability and reprdducibility ofthe electrode were tested and the pyridoxine samplcs were analyzed by diractpotcntiometry and potentiometric titration procedures. The pKvalue of pyridoxinewas determined potentio-metrically by using the proposed pyridoxine electrode.