Abstract: The specific retention volumes of some solutes in some binary stationary liquid employed as couple columns, heterogenous packing columns and homogeous packing columns have been measured. By using the Flory-Huggins equation for ternary system and the values of Flory-Huggins interaction parameter characterizing interactions between two different solvent molecules, the cause of the difference of the retention behavior between these three kinds of packing column have been revealed from the point of view of thermodynamics aspect.