Abstract: In present paper, the results of analysis of non-volatile constituents of roots of "Min-gui" of Gansu Province, Angelica sinensis (Oliv.) Diels, have been reported. Thirty seven organic constituents have been identified, of which fifteen have not yet been found in Angelica sinensis before, including an optically active new compound named as Angelicide and an antibiotic Brefeldin A. The structure of Angelicide has been determined by IR, high resolution MS, ¹H NMR, ¹³C NMRand single crystal X-ray diffraction. By plasma emission spectrometry, thirteen minor elements have been detected.