CJCU

Chem. J. Chinese Universities ›› 1982, Vol. 3 ›› Issue (S1): 21.

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STUDY ON THE INTERACTION OF CISPLATIN WITH NUCLEOSIDES BY 13C NMR

Tang Wenxia, Yuan Chuanrong, Zhang Shiyong, Tai Anpang   

  1. Cordination Chemistry Institute, Nanjing University, Nanjing
  • Received:1982-05-20 Online:1982-12-31 Published:1982-12-31

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Abstract:

Interaction of cisplatin with thymidine, cytidine, guanosine and 5'-ade-nosine phosphate in aqueous solution was studied by 13C NMR. The formation of a series of complexes and the site of complexation of nucleo-sides (or nucleotide) to cisplatin in the reaction systems were ascertained, It was found that bonding occurred at N3 in thymidine and cytidinp with the formation of complexes, cis-[Pt(NH3)2(N3-Thy H-1)2] and cis-[Pt(NH3)2(N3-Cyt)3]2+. In neutral solution, cisplatin reacted with guanosine at N7 or/and N7' N1 according to the mol ratio of the reactants forming cis-[Pt(NH3)2(N7-Guo)3]2+ and [Pt(NH3)2 (N7' N1-GuoH-1)]nn+, with a minute amount of [Pt(NH3)2(N7'O(C6)-Guo)]2+ when pH=3. With 5'AMP, cisplatin formed cis-[Pt(NH3)2(N75'-AMP)2]2- or/and cis-[Pt(NH3)3 (N7, N1-5'-AMP)]n in accordance with the mol ratio of the reactants.On the basis of the above findings, the possible mode of reaction of cisplatin with DNA in the cancer cells is discussed. It is probable that Jntrastrand cross linking is formed between cisplatin and DNA through N7, N1 atoms of two neighbouring guanines, which may cause lesion of the cancer cells.

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