Abstract: Forty-two ferrocene derivatives (including hydrocarbons,ethers,aldehydes,ketones,alcohols,amines,benzenesulfonamides,carboxylic acids,and acrylonitriles) have been separated by thin-layer chroma-tography using silica gel as adsorbent,and toluene-petroleum ether--acetone (15:1:1 or 15:1:3),benzene-alcohol (15:1) or toluene-petroleum ether-alcohol(15:1:1) as development.The following rules have been obtainedⅠ.The relations between the structures of the compounds and their Rs values:1.The Rs values decrease with the increase of the polarity of the substituents of the same series.2.The Rs values decrease with the increase of the number of the substituents of the same type.The Rs values of the monosubsti tuted coinpounds>those of the disubstituted ones.Ⅱ.The Rs values increase with the increase of the polarity of the development.The fact that some ferrocenes derivatives containing side chain hydroxy-group do not obey the rules mentioned above has been explained by means of their IRand NMRspectra.