Shear-Induced Gelation Kinetics and Mechanisms of Silk Fibroin Hydrogels

doi:10.7503/cjcu20250327

Chem. J. Chinese Universities

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Shear-Induced Gelation Kinetics and Mechanisms of Silk Fibroin Hydrogels

LU Jialin^1,2，ZHUANG Yaling^1,3, WANG Yanan^1,3, CHANG Fei², DING Jianxun^1,3, CHEN Xuesi^1,3

1. State Key Laboratory of Polymer Science and Technology,Changchun Institute of Applied Chemistry, Chinese Academy of Sciences 2. Department of Foot and Ankle Surgery,The Second Hospital of Jilin University 3. School of Applied Chemistry and Engineering,University of Science and Technology of China

Received:2025-11-02 Revised:2025-12-06 Online:2025-12-10 Published:2025-12-10
Contact: Jian-Xun DING E-mail:jxding@ciac.ac.cn
Supported by:
Supported by the National Key R&D Program of China(No.2022YFE0107700), the National Natural Science Foundation of China(Nos.U21A2099 and 82572761) and the Science and Technology Development Program of Jilin Province, China(No.20240101002JJ)

Abstract

Abstract: In this study, a shear-heating method was employed to induce the formation of silk fibroin hydrogels enrich in β-sheet structures. The effects of shear duration, incubation temperature, and protein concentration on the kinetics of β-sheet assembly and the mechanical properties of the resulting hydrogels were systematically investigated. The results demonstrated that a 0.03 g mL-1 silk fibroin solution subjected to shear at 10000 r min-1 for 10 min and followed by incubation at 60 °C completed gelation within 30 min, yielding a physically cross-linked network containing 63.51% β-sheet structures, with a storage modulus of 25.70 kPa and a compressive strength of 108.29 kPa. Furthermore, the self-assembly kinetics of the molecular chain transition from random coils to β-sheet structures and the corresponding evolution of mechanical properties were elucidated. This work provides optimized processing strategies and experimental evidence for the preparation of shear-induced silk fibroin hydrogels and supports their potential applications in tissue engineering.

Key words: Silk fibroin hydrogel, Shear-inducing, Secondary structure transition, Gelation kinetics, Biomedical material

CLC Number:

O614.8

TrendMD:

LU Jialin, ZHUANG Yaling, WANG Yanan, CHANG Fei, DING Jianxun, CHEN Xuesi. Shear-Induced Gelation Kinetics and Mechanisms of Silk Fibroin Hydrogels[J]. Chem. J. Chinese Universities, doi: 10.7503/cjcu20250327.

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Shear-Induced Gelation Kinetics and Mechanisms of Silk Fibroin Hydrogels

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