Chem. J. Chinese Universities ›› 2017, Vol. 38 ›› Issue (8): 1368.doi: 10.7503/cjcu20170127

• Organic Chemistry • Previous Articles     Next Articles

Detection of Animal Tissue Distribution of β-1,3/1,6-Glucan from Durvillaea antarctica by in vivo Imaging

ZHAO Xiaoliang, LIU Xi, YANG Yi, CAI Chao, LÜ Youjing, LI Guoyun, HAO Jiejie, YU Guangli   

  1. Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
  • Received:2017-03-02 Revised:2017-06-02 Online:2017-08-10 Published:2017-06-02
  • Supported by:

    Supported by the National Natural Science Foundation of China(No. 31670811), the Major Projects of Science and Technology in Shandong Province, China(No. 2015ZDJS04002) and the Program for Taishan Scholar of Shandong Province, China.

Abstract:

In the present study, β-1,3/1,6-glucan(DAG) from Durvillaea antarctica was investigated. First, the functional amino group was introduced into the DAG reduction site by reductive amination reaction. The reaction product was then coupled with NHS-activated fluorescent dye(Cy7), and the Cy7-labeled DAG molecule was obtained subsequently. The distribution of DAG in mice was studied by small animal in vivo imaging technique. The results show that DAG is mainly distributed in the lungs, livers and kidneys, which possessed the characteristics of targeting the mouse lungs and excreting through the kidneys. The method is simple and reliable, which provides an important reference for the distribution and metabolism study of polysaccharides and oligosaccharides in vivo.

Key words: β-1,3/1,6-Glucan, Fluorescence labeling, In vivo imaging, Tissue distribution

CLC Number: 

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