MWCNTs-rGO/PDDA-AuNPs Nanocomposite Modified Electrode for Sensitive Detection of Ractopamine†

doi:10.7503/cjcu20160026

Abstract

Abstract:

A nanocomposite film of MWCNTs-rGO/PDDA-AuNPs was fabricated through self-assembly method. The surface morphologies of different composite modified glassy carbon electrodes(GCEs) were characterized by means of TEM and UV-Vis spectroscopy, and the cyclic voltammetry behaviors of ractopamine(Rac) were investigated. The results showed that the MWCNTs-rGO/PDDA-AuNPs composite exhibited significantly electrocatalytic activity towards oxidation of Rac. The results of differential pulse voltammetry showed that the electrode possessed a linear range of 0.036—4.5 μmol/L and a detection limit of 6.35 nmol/L(S/N≥3) with good stability, reproducibility and selectivity. The electrode can be well applied to determination of Rac in pork serum and pork urine with a recovery rate of 95.4%—105.9%, suggesting a potential application for actual samples in food security field.

Key words: Ractopamine, Reductive graphene oxide, Poly(diallyldimethylammonium chloride), MWCNTs-rGO/PDDA-AuNPs composite, Food safety inspection

CLC Number:

TrendMD:

XUN Yan, CAO Zhong, SONG Tianming, LÜ Chaozhi, LIU Feng, HE Jinglin, YANG Ronghua. MWCNTs-rGO/PDDA-AuNPs Nanocomposite Modified Electrode for Sensitive Detection of Ractopamine^†[J]. Chem. J. Chinese Universities, 2016, 37(5): 835.

Figures/Tables 10

Fig.1 TEM images of PDDA-AuNPs(A) and MWCNTs-rGO/PDDA-AuNPs hybrids(B)

Fig.2 UV-Vis absorption spectra of GO(a), PDDA-AuNPs(b), MWCNTs-GO(c), MWCNTs-rGO(d) and MWCNTs-rGO/PDDA-AuNPs hybrids(e)

Fig.3 Q-t curves for the bare GCE(a), MWCNTs-rGO/GCE(b) and MWCNTs-rGO/PDDA-AuNPs/GCE(c) in 0.2 mmol/L K3[Fe(CN)6] with pulse width of 0.25 s

Fig.4 CV curves for the oxidation of Rac at bare GCE without(a) and with(b) 3.6×10-6 mol/L Rac, and at PDDA-AuNPs/GCE(c), MWCNTs-rGO/GCE(d) and MWCNTs-rGO/PDDA-AuNPs/GCE(e) in PBS(0.01 mol/L, pH=6.5) containing 3.6×10-6 mol/L RacScan rate: 50 mV/s.

Fig.5 Effects of pH on the oxidation peak current(A) and peak potential(B) of 2.64×10-6 mol/L Rac in 0.01 mol/L PBS

Fig.6 CV curves of MWCNTs-rGO/PDDA-AuNPs/GCE responding to Rac at different scan rates(A), plots of peak current vs. scan rate(B) and peak potential vs. logarithmic scan rate(C)Scan rate/(mV·s-1): a. 10; b. 25; c. 50; d. 75; e. 100; f. 125.

Fig.7 Schematic diagram for preparation of MWCNTs-rGO/PDDA-AuNPs/GCE modified GCE and its detecting of Rac

Fig.8 DPV curves of MWCNTs-rGO/PDDA-AuNPs/GCE responding to different concentrations of Rac in 0.01 mol/L PBS(pH=6.5)(A) and linear plot of response current vs. concentration(B)c/(mol·L-1): a. 3.6×10-8; b. 6.60×10-8; c. 3.96×10-7; d. 7.92×10-7; e. 1.45×10-6; f. 2.90×10-6; g. 3.60×10-6; h. 4.50×10-6. Accumulation potential: -0.3 V, accumulation time: 120 s.

Fig.9 Effect of interferring substance on the modified electrodea. Rac; b. L-Leucine; c. L-Cysteine; d. L-Arginine; e. L-Alanine; f. L-Glycine; g. ascorbic acid; h. I-; i. NO3-; j. SO42-; k. K+; l. Zn2+; m. Cle; n. Sal; o. Pea.

Table 1 Application of MWCNTs-rGO/PDDA-AuNPs/GCE for determination of Rac in pork serum and urine samples

Sample	No.	Spiked/(μmol·L^-1)	Found/(μmol·L^-1)	Recovery(%)	RSD(%)
Pork serum	1	0.1850	0.1960	105.9	1.9
	2	0.6000	0.6055	100.9	3.4
	3	1.180	1.166	98.8	4.2
	4	1.980	2.019	102.0	5.8
	5	3.500	3.547	101.3	5.7
Pork urine	1	0.1850	0.1780	96.2	1.3
	2	0.6000	0.5722	95.4	2.3
	3	1.180	1.197	101.4	3.4
	4	1.980	1.937	97.8	4.1
	5	3.500	3.598	102.8	4.2

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