Preparation of Biocompatible Protein-hydrogel-based Micro-patterns via UV Lithography†

doi:10.7503/cjcu20150733

Abstract

Abstract:

Through facile UV lithography, without chemical modification, biocompatible natural protein-hydrogel-based film and micro-patterns were prepared. The micro-patterns can be used as micro-grating optical devices. Then rainbow feature was observed and diffraction fringe for a 638 nm laser beam was obtained under white light, even with a diffraction level up to 11. Further more, micro-wrinkles could be controlled by changing related parameters. Finally, HepG-2 cells were cultured on the protein micro-patterns and cell micro-patterns were obtained.

Key words: UV Lithography, Protein hydrogel, Micro-grating, Bio-compatibility, Micro-wrinkle

CLC Number:

O644.11

TrendMD:

ZHENG Boyuan, JI Fengying, HOU Zhishan, SUN Siming, SUN Yunlu, YANG Ruizhu. Preparation of Biocompatible Protein-hydrogel-based Micro-patterns via UV Lithography^†[J]. Chem. J. Chinese Universities, 2016, 37(4): 715.

Figures/Tables 8

Fig.1 Spectrum of the UV light source(A) and diagram of UV-lithographing protein gratings(B)

Fig.2 Optical microscopic images of protein gratings^The insets are the grating masks for UV-lithographing. Period/μm: (A) 55; (B) 25; (C) 15; (D) 10.

Fig.3 Optical characterization of the micro-gratings ^(A)—(D) Rainbow images shown by the protein-based micro-patterns under white light source; (E) Protein-based micro-pattern as micro-grating optical device(diffraction fringes of a 638 nm laser beam). The inset shows the diffractive optical testing system.

Scheme 1 Several possible reactions of histidine-involved protein photo-crosslinking

Fig.4 AFM images of micro-gratings^Period/μm: (A) 10; (B) 15; (C) 25; (D) 55.

Fig.5 Smooth protein hydrogel micro-stripe structure via optimized UV-lithographing^(A) AFM image of the smooth protein hydrogel micro-structure; (B) AFM 3D profile of the structure in (A); (C) the cross-section profile at position marked by the line in (A).

Fig.6 Cell micropatterning via the protein microstructures^(A), (B) Optical microscopic images of HepG-2 cells cultured on the protein micro-patterns; (C) SEM image of patterned cells on protein microstructures; (D) cross-section SEM image of patterned cells on protein microstructures.

Fig.7 3D AFM profile of a cell guided along protein-hydrogel microstructure

References 38

[1]	Llobera1 A., Juvert J., González-Fernández1 A., Ibarlucea1 B., Carregal-Romero1 E., Büttgenbach S., Fernández-Sánchez C., Light: Science & Applications, 2015, 4(4), e271
[2]	Li N., Tittl A., Yue S., Giessen H., Song C., Ding B. Q., Liu N., Light: Science & Applications, 2014, 3, e226
[3]	Deming T. J., Advanced Materials, 1997, 9(4), 299—311
[4]	Felix J. M., Gatenholm P., Journal of Applied Polymer Science, 1991, 42(3), 609—620
[5]	Hu T., Kaplan D. L., Omenetto F. G., Advanced Materials, 2012, 24(21), 2824—2837
[6]	Oh H. H., Ko Y. G., Lu H. X., Kawazoe N., Chen G. P., Advanced Materials, 2012, 24(31), 4311—4316
[7]	Sun Y.L., Sun S. M., Wang P., Dong W. F., Zhang L., Xu B. B., Chen Q. D., Tong L. M., Sun H. B.,Small, 2015, 2869—2876
[8]	Bar-Noam A. S., Farah N., Shoham S., Light: Science & Applications, 2016, 5(1), e16007
[9]	Hong S., Lee M. Y., Jackson A. O., Lee L. P., Light: Science & Applications, 2015, 4, e267
[10]	Liang K., Ricco R., Doherty C. M., Styles M. J., Bell S., Kirby N., Mudie S., Haylock D., Hill A. J., Doonan C. J., Falcaro P., Nature Communications, 2015, 6(8240), 1—8
[11]	Pincella F., Isozaki K., Miki K., Light: Science & Applications, 2014, 3(1), e133
[12]	Urness A. C., Moore E. D., Kamysiak K. K., Cole M. C., Mcleod R. R., Light: Science & Applications, 2013, 2(3), e56
[13]	Calafiore G., Koshelev A., Dhuey S., Goltsov A., Sasorov P., Babin S., Yankov V., Cabrini S., Peroz C., Light: Science & Applications, 2014, 3, e203
[14]	Zhang W., Zappe H., Seifert A., Light: Science & Applications, 2014, 3(2), e145
[15]	Kawata S., Sun H. B., Nature, 2001, 412(6848), 697—698
[16]	Sugioka K., Cheng Y., Light: Science & Applications, 2014, 3(4), e149
[17]	Wu D., Xu J., Niu L. G., Wu S. Z., Midorikawa K., Sugioka K., Light: Science & Applications, 2015, 4(4), e228
[18]	Wang C., Zhu F., Ren L., Li A. W., Chen C., Yang R., Guo J. C., Xue Y., Zhang X. Y., Zhu C. C., Chen Q. D., Yu Y. S., Chem. Res. Chinese Universities, 2013, 29(6), 1199—1202
[19]	Xiong W., Zhou Y. S., He X. N., Gao Y., Mahjouri-Samani M., Jiang L., Baldacchini T., Lu Y.F., Light: Science & Applications, 2012, 1, e6
[20]	Kumar K., Lee K. K., Li J., Nogami J., Kherani N. P., Herman P. R., Light: Science & Applications, 2014, 3(1), e157
[21]	Vannahme C., Dufva M., Kristensen A., Light: Science & Applications, 2015, 4(4), e269
[22]	Gou M.L., Qu X., Zhu W., Xiang M. L., Yang J., Zhang K., Wei Y. Q., Chen S. C., Nature Communications, 2014, 5(4774), 1—9
[23]	Xia H., Wang J., Tian Y., Chen Q. D., Du X. B., Zhang Y. L., He Y., Sun H. B., Advanced Materials, 2010, 22, 3204—3207
[24]	Yang J., Luo F. F., Kao T. S., Li X., Ho G. W., Teng J. H., Luo X. G., Hong M. H., Light: Science & Applications, 2014, 3(7), e185
[25]	Zhang Y. L., Xia H., Kim E., Sun H. B., Soft Matter, 2012, 8, 11217—11231
[26]	Wu D., Wu S. Z., Chen Q. D., Zhang Y. L., Yao J., Yao X., Niu L. G., Wang J. N., Jiang L., Sun H. B., Advanced Materials, 2011, 23, 545—549
[27]	Liebetraut P., Petsch S., Liebeskind J., Zappe H., Light: Science & Applications, 2013, 2(9), e98
[28]	Wu D., Chen Q. D., Niu L. G., Wang J. N., Wang J., Wang R., Xia H., Sun H. B., Lab. Chip., 2009, 9, 2391—2394
[29]	Zhang Y. L., Guo L., Wei S., He Y. Y., Xia H., Chen Q. D., Sun H. B., Xiao F. S., Nano Today, 2010, 5, 15—20
[30]	Guo L., Jiang H. B., Shao R. Q., Zhang Y. L., Xie S. Y., Wang J. N., Li X. B., Jiang F., Chen Q. D., Zhang T., Sun H. B., Carbon, 2012, 50(4), 1667—1673
[31]	Sun Y. L., Dong W. F., Yang R. Z., Meng X., Zhang L., Chen Q. D., Sun H. B., Angew. Chem. Int. Edit., 2012, 51, 1558—1562
[32]	Sun Y. L., Dong W. F., Niu L. G., Jiang T., Liu D. X., Zhang L., Wang Y. S., Chen Q. D., Kim D. P., Sun H. B., Light: Science & Applications, 2014, 3, e129
[33]	Kurland N. E., Dey T., Kundu S. C., Yadavalli V. K., Advanced Materials, 2013, 25, 6207—6212
[34]	Shen H. R., Spikes J. D., Smith C. J., Kopecek J., Journal of Photochemistry and Photobiology A: Chemistry, 2000, 130(2000), 1—6
[35]	Fancy D. A., Kodadek T., Proceedings of the National Academy of Sciences of the United States of America, 1999, 96, 6020—6024
[36]	Kochevar I. E., Lynch M. C., Zhuang S. G., Lambert C. R., Photochem. Photobiol., 2000, 72, 548—554
[37]	Chan E. P., Crosby A. J., Advanced Materials, 2006, 18, 3238—3242
[38]	Wang J. M., Wang C., Wang M. C., Jiang L., Chem. J. Chinese Universities, 2011, 32(8), 1807—1811
	(王景明, 王春, 王明超, 江雷, 高等学校化学学报, 2011, 32(8), 1807—1811)