Preparation of Up-conversion Luminescent Material Labeled Antibody and Its Application in Immunohistochemistry

doi:10.7503/cjcu20120667

Abstract

Abstract:

The up-conversion luminescent material for optically written display, NaGdF₄: Yb³⁺,Er³⁺ up-conversion nanoparticies(UCNPs) were prepared under microwave irradiation. The particle size was about 65 nm and the samples exhibited green(550 nm) fluorescence excited by a 980 nm diode laser. The UCNPs@SiO₂-NH₂ nanocomposites, the size of which was 70 nm, were prepared and conjugated with polyclonal antibidy. Then, the ability of polyclonal antibody fluorescent probes was detected to recognize the expression of tissue inhibitor of metalloproteinases-4(TIMP-4) in the endometrial gland cells by immunohistochemistry. The results show that the as-prepared UCNPs have uniform shape and size distribution. The UCNPs@SiO₂-NH₂ nanocomposites offer some distinct advantages, such as good distribution and water-solubility, strong and stable upcoversion fluorscence as well as significant auto-fluorescence from biological tissues resting in low signal to background ratio, and are potentially to apply to detect the expression of protein in tissues at 980 nm excitation.

Key words: Up-conversion luminescence, Rare-earth fluoride nanoparticles, Immunohistochemistry, Polyclonal antibody

CLC Number:

O614.33

TrendMD:

CHEN Ying, LI Fei-Fei, LI Chun-Guang, PAN Qing-Zhi, CUI Man-Hua. Preparation of Up-conversion Luminescent Material Labeled Antibody and Its Application in Immunohistochemistry[J]. Chem. J. Chinese Universities, 2013, 34(4): 788.

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