Chem. J. Chinese Universities ›› 2013, Vol. 34 ›› Issue (2): 272.doi: 10.7503/cjcu20120350
Previous Articles Next Articles
LIU Yan, WU Qin, ZHENG Yu-Chuan, LIU Yang-Zhong
Received:
Online:
Published:
Abstract:
The abilities of five Pd complexes, including Pd(bipy)Cl2, Pd(phen)Cl2, [Pd(dien)Cl]Cl, trans-Pd(NH3)2Cl2 and cis-Pd(NH3)2Cl2, to inhibit protein splicing of Mycobacterium tuberculosis RecA intein have been evaluated. The results showed that trans-Pd(NH3)2Cl2 has the best inhibition efficiency(IC50=3.3×10-5 mol/L). Competition experiments suggest that the inhibition of protein splicing can be ascribed to the interaction between Pd complexes and the first residue(cysteine) of RecA intein. Furthermore, the difference in the fluorescence quenching of ΔΔIhh-CM by Pd complexes is most likely due to the ligand steric hindrance.
Key words: Intein, Protein splicing, Pd complex
CLC Number:
O614.82
TrendMD:
LIU Yan, WU Qin, ZHENG Yu-Chuan, LIU Yang-Zhong. Inhibition of Protein Splicing of Mycobacterium tuberculosis RecA intein by Pd Complexes[J]. Chem. J. Chinese Universities, 2013, 34(2): 272.
0 / / Recommend
Add to citation manager EndNote|Ris|BibTeX
URL: http://www.cjcu.jlu.edu.cn/EN/10.7503/cjcu20120350
http://www.cjcu.jlu.edu.cn/EN/Y2013/V34/I2/272
