酸性接枝淀粉固定化糖化酶及稳定性研究

高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (8): 1346.

酸性接枝淀粉固定化糖化酶及稳定性研究

吴永革, 葛玉斌, 孙文田, 王树岩, 周慧, 李惟

吉林大学分子生物学系, 长春, 130023

收稿日期:1997-06-09 出版日期:1998-08-24 发布日期:1998-08-24
通讯作者: 吴永革,男,32岁,博士研究生,讲师.
作者简介:吴永革,男,32岁,博士研究生,讲师.
基金资助:
国家自然科学基金(批准号:2947159)资助课题.

Preparation and Stability of Glucoamylase Immobilized on Porous Starch Graft Copolymer Beads

WU Yong-Ge, GE Yu-Bin, SUN Wen-Tian, WANG Shu-Yan, ZHOU Hui, LI Wei

Department of Molecular Biology, Jilin University, Changchun, 130023

Received:1997-06-09 Online:1998-08-24 Published:1998-08-24

摘要/Abstract

摘要： 糖化酶(EC.3.2.1.3,GA)是工业上应用规模最大的3种酶制剂之一.目前,工业生产中大多采用游离GA,这给产物的分离和纯化带来诸多不便.因此,采用固定化糖化酶(IGA)是酶制剂工业发展的必然趋势.

关键词: 多孔球状淀粉接枝共聚物, 固定化糖化酶, 酶的稳定性

Abstract: Glucoamylase from Aspergillus niger(EC3.2.1.3) was immobilized on porous starch graft copolymer beads (PSGC) which were synthesized in our laboratory. Some of the factors which influence the activity of the immobilized enzyme and the stability of the immobilized enzyme were investigated. The activity and special activity is 1340 IUper gram dry gel and 58.1 IUper millgram protein respectively when GA was immobilized under best condition found by ourselves. The optimium pHof the immobilized glucoamylase shifted 1.2 unit towards basic direction as compared to that of soluble enzyme. The thermostability of immobilized glucoamylase is longer than that of soluble enzyme, the operational half life of immobilized glucoamylase was 28 d and 8d with 20% dextrin as substrate at 55 ℃ and 60 ℃, respectively.

Key words: Porous starch graft copolymer beads, Immobilized glucoamylase, Enzyme stability

中图分类号:

O631

TrendMD:

吴永革, 葛玉斌, 孙文田, 王树岩, 周慧, 李惟. 酸性接枝淀粉固定化糖化酶及稳定性研究. 高等学校化学学报, 1998, 19(8): 1346.

WU Yong-Ge, GE Yu-Bin, SUN Wen-Tian, WANG Shu-Yan, ZHOU Hui, LI Wei. Preparation and Stability of Glucoamylase Immobilized on Porous Starch Graft Copolymer Beads. Chem. J. Chinese Universities, 1998, 19(8): 1346.